Composite

Part:BBa_K3338019:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


IL-6 Pmut-hGLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Design Notes

The interleukin-6 promoter is compatible with the RFC10 assembly standard.


Source

The human interleukin-6 promoter is mutagenized to make it compatible with the RFC10 assembly standard. hGLuc is a human codon optimized form of Gaussia luciferase from Gaussia princeps.

References